Histopathological Evaluation of the Effects of Low-level Laser Therapy on the Healing of Experimental Cryosurgical Wound in Normal Rat Skin
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Abstract
Introduction: This research aimed to study the healing process of cryosurgical wounds after topical application of low-level laser and to evaluate its effects for the prevention of healing complications.
Materials and Methods: A total of 50 healthy male Wistar rats with an average weight of 200-250 g were selected and randomly divided into two main groups. In control group (N=25), cryosurgery was done without using of low-level laser. In experimental group (N=25), cryosurgery was done and the low-level laser was used for 10 days post-surgery. Each main group was divided into five subgroups (n=5) according to histopathological evaluation date (3, 7, 14, 21 and 28 days post-surgical) and healing process was evaluated on the wound specimens harvested at above-mentioned days. The full- thickness wounds were harvested in 40 mm in length and 20 mm in width and the specimens were stained using hematoxylin-eosin and Masson’s Trichrome staining. The healing process was evaluated and compared in groups by observation of granulation tissue and collagen fibers amounts, epithelial gap size, the number of inflammatory cells, and the rate of angiogenesis. Data were collected and statistically analyzed and compared between the experimental and control groups on specific days using the Kruskal-Wallis and Dan post hoc statistical tests.
Results: The obtained data were compared between the experimental and control groups on different days using the Kruskal-Wallis and Dan post hoc statistical tests. The statistical results revealed significant differences between groups (P<0.05) in all mentioned variables. It revealed that the healing process in the experimental group took place at least one week earlier than the control group.
Conclusion: The results of this animal study showed that the low-level laser therapy on an experimental cryosurgical wound not only accelerates the healing process but also can be effective in the prevention of healing complications. The authors suggest further research to extend the results to human applications.
Ablin RJ. Handbook of cryosurgery (Science and practice of surgery) . New York: Marcell Decker Inc.; 1980. https://www.google.com/books/edition/Handbook_of_Cryosurgery/yTVsAAAAMAAJ?hl=en
Mohit Mafi S, AnissianA, Anissian A. [Evaluation of injury and healing process after cryogenic partial nephrectomy (Persian)]. Pajoohande. 2011; 16(1):18-23. http://pajoohande.sbmu.ac.ir/browse.php?a_id=1078&sid=1&slc_lang=fa
Shepherd JP, Dawber RP. Wound healing and scarring after cryosurgery. Cryobiology. 1984; 21(2):157-69 [DOI:10.1016/0011-2240(84)90207-4]
Ezzati k. [Clinical Laser Therapy (Persian)]. Tehran: Setayesh Hasti Publishing; 2018. https://www.gisoom.com/book/11470899/%DA%C-1/
Baxter GD, Diamantopoulos C. Therapeutic lasers: Theory and practice. New York: Churchill Livingstone; 1994. https://books.google.nl/books?id=Mc0ivgAACAAJ&dq=Therapeutic+Lasers+:+Theory+and+Practice&hl=en&sa=X&redir_esc=y
Lafta Mezaal A, Bashardoust Tajali S, Olyaei Gh, Jalaie Sh, Alwatifi ST. Effects of low-level laser versus laser acupuncture in patients with knee osteoarthritis: A randomized controlled trial. Journal of Modern Rehabilitation. 2018; 12(3):181-94. [DOI:10.32598/JMR.V12.N3.181]
Kahn J. Principles and practice of electrotherapy. New York: Churchill Livingstone; 1987. https://books.google.nl/books?id=DwFtAAAAMAAJ&q=Kahn+J.+Principles+and+Practice+of+Electrotherapy&dq=Kahn+J.+Principles+and+Practice+of+Electrotherapy&hl=en&sa=X&redir_esc=y
Mester AF, Mester A. Wound healing. Laser Therapy. 1989; 1(1):7-15. [DOI:10.5978/islsm.89-RE-01]
Mester E, Mester AF, Mester A. The biomedical effects of lase application. Lasers in Surgery and Medicine. 1985; 5(1):31-9. [DOI:10.1002/lsm.1900050105] [PMID]
Schindl A, Schindl M, Pernerstorfer-Schön H, Schindl L. Low-intensity laser therapy: A review. Journal of Investigative Medicine. 2000; 48(5):312-26. [PMID]
Baxter GD, Walsh DM, Allen JM, Lowe AS, Bell AJ. Effects of low intensity infrared laser irradiation upon conduction in the human median nerve in vivo. Experimental Physiology. 1994; 79(2):227-34. [DOI:10.1113/expphysiol.1994.sp003755] [PMID]
Bjordal JM, Lopes-Martins RA, Iversen VV. A randomized, placebo controlled trial of low level laser therapy for activated Achilles tendinitis with microdialysis measurement of peritendinous rostaglandin E2 concentrations. British Journal of Sports Medicine. 2006; 40(1):76-80. [DOI:10.1136/bjsm.2005.020842] [PMID] [PMCID]
Campana VR, Castel A, Vidal AE, Juri H, Palma JA. Prostaglandin E2 in experimental arthritis of rats irradiated with He-Ne laser. Journal of Clinical laser Medicine & Surgery. 1993; 11(2):79-81. [DOI:10.1089/clm.1993.11.79]
Chung H, Dai T, Sharma SK, Huang YY, Carroll JD, Hamblin MR. The nuts and bolts of low-level laser (light) therapy. Annals of Biomedical Engineering. 2012; 40(2):516-33 [DOI:10.1007/s10439-011-0454-7] [PMID] [PMCID]
Lubart R, Eichler M, Lavi R, Friedman H, Shainberg A. Low-energy laser irradiation promotes cellular redox activity. Photomedicine and Laser Surgery. 2005; 23(1):3-9. [DOI:10.1089/pho.2005.23.3] [PMID]
Ramsey I. Small Animall Formulary. Gloucester: BSaVA; 2011. http://ndl.ethernet.edu.et/handle/123456789/2026
Medrado AR, Pugliese LS, Reis SR, Andrade ZA. Influence of low level laser therapy on wound healing and its biological action upon myofibroblasts. Lasers in Surgery and Medicine. 2003; 32(3):239-44. [DOI:10.1002/lsm.10126] [PMID]
Kazemikhoo N, Khamse ME, Hashem Dabaghian F, Hajizadeh M, Akrami M. [Evaluation of the effects of low power laser in the treatment of grade 2 and 3 diabetic foot ulcers(Persian)]. Iranian Journal of Diabetes and Metabolism. 2008; 8(2):195-202. http://ijdld.tums.ac.ir/article-fa.html
Calin MA, Coman TO, Calin MR. The effect of low level laser therapy on surgical wound healing. Romanian Reports in Physics. 2010; 62(3):617-27. https://www.researchgate.net/profile/Romeo-Calin/publication/228621606_The_effect_of_low_level_laser_therapy_on_surgical_wound_healing/links/00b49518938ea411e2000000/The-effect-of-low-level-laser-therapy-on-surgical-wound-healing.pdf
Melo VA, Anjos DC, Albuquerque Júnior R, Melo DB, Carvalho FU. Effects of low laser on sutured wound healing in rats. Acta Cirúrgica Brasileira. 2011; 26(2):129-34. [DOI:10.1590/S0102-86502011000200010] [PMID]
| Files | ||
| Issue | Vol 15 No 3 (2021) | |
| Section | Research Article(s) | |
| DOI | https://doi.org/10.18502/jmr.v15i3.7734 | |
| Keywords | ||
| Cryosurgery Low-level Laser Rat Skin Healing | ||
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