Tehran University of Medical Sciences Journal of Modern Rehabilitation 2538-385X 0 - 2025 12 07 1450 1450 Jayashree Duraimurugan Saveetha College of Physiotherapy, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India-602105 Shenbaga Sundaram Subramanian Saveetha College of Physiotherapy, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India-602105 Mohamed Sahal Saveetha College of Physiotherapy, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India-602105 Diovin Derose Vianni Saveetha College of Physiotherapy, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India-602105 Eunice Keren Singarayar Sri Ramakrishna College of Physiotherapy, Sri Ramakrishna Institute of Paramedical Sciences (SRIPMS), Coimbatore, Tamil Nadu, India-641044 Fadwa Alhalaiqa Department of Nursing, Qatar University, Doha, Qatar 2025 10 19 2025 12 07 Background: Low back pain (LBP) is a musculoskeletal disorder commonly associated with altered gait patterns, but little information exists on comparative specific spatiotemporal gait parameters in individuals with LBP. This gap is addressed in the present study by comparing the mean values of stride length, step length, and stride time, as well as corresponding measures of hip extension and peak knee flexion angle, using Kinovea software. Material and Methods: This observational study was done on 200 subjects, 100 healthy (group I) and 100 LBP individuals (group II). The inclusion and exclusion criteria were used to select the study subjects. All participants underwent a gait analysis using Kinovea software, and gait parameters, including stride length, step time, and hip extension, were also measured. Statistical associations with gait parameters and the presence of LBP were analyzed. Results: Subjects with low back pain showed significant gait alteration with decreased stride length (mean difference (MD) 32.93 cm, p=0.001), decreased step length (18.52 cm, p=0.001), and reduced hip extension (1.88°, p=0.001) with weak to moderate correlation (r=0.214-0.282). These gait impairments were independent of the body composition. Conclusions: The research results in the identification of significant changes in the gait patterns of patients with and without low back pain, as revealed by Kinovea software, characterized by low step length, high temporal duration, and low magnitude of hip extension. These results provide quantitatively measurable differences in gait dynamics due to inter-individual differences that are observed clinically. https://jmr.tums.ac.ir/index.php/jmr/article/view/1450